2.5. Proximate analysis and wet gluten content
The proximate composition of the barnyard millet and muffin
samples (control, BMF muffins) was analyzed using the AOAC (2000)
methods: moisture (Method 925.09), crude fat (Method 969.24),
protein (Method 950.48, Nx6.25), and total minerals (method
923.03). Crude fiber was estimated according to ISO 5498:1981. The
muffin was crumbled, mixed uniformly and portion of the mixed
material was taken to represent the whole muffin. Three muffin
samples were used as replicate each time. The wet gluten content of
the barnyard millet flour was assessed by mechanical means using
the ISO 7495:1990 method.