The diluted RBCs were mixed with double strength Drabkin’s reagent
to inhibit the peroxidase activity of haemoglobin and then
used for GPx assays using the Ransel kit (Randox Laboratories
Ltd., Northern Ireland, UK). The specific activity for GPx was expressed
in units per gram of haemoglobin (U/g Hb). The method
used was adopted from one previously described by Paglia and Valentine
(1967) with some modifications