After 2 h of incubation, pancreatic and bile fluid were supplemented to the gastric digest to initiate the small in- testine incubation (at pH 6.5). Small intestine incubation of 4 h of Butyricicoccus that had been acid stressed (pH 2 and pH 3 during stomach phase) resulted in Butyricicoccus levels of 3.4 ± 0.7 an