Casein enzymic hydrolysate provides essential nutrients to the organisms. Dextrose serves as an energy source by being the
fermentable carbohydrate while bromo cresol purple is a pH indicator. Acid producing organisms produce yellow colonies.
The plates should be incubated at 55°C for 48 hours in a humid incubator.
While using the agar media, serially diluted test sample are mixed with the media in sterile Petri dishes. Standard procedures
issued by various associations should be followed for testing of samples.