14. Transfer the media to an autoclavable recipient (bottle or erlen) with a capacity of at least 200 ml larger than the quantity of liquid it contains. This will prevent media
loss/splash in the autoclave chamber during the autoclave cycle. When using a bottle, close the cap loosely (to avoid pressurization).
15. Once the autoclave cycle is completed, take out the recipient and dispense the media into tubes while still hot (still liquid). Note that adequate number of tubes + caps must be autoclaved prior media distribution. Distribution is performed under laminar flow (sterile conditions) using sterile pipette. This step can be semi automatized by using an automatic pipetter. Distribute 5 ml per tube.