To determine the expression pattern of OsBADH1,
we isolated the total RNAs from various rice tissues
grown in different conditions (Table I) and analyzed the
transcription by reverse transcription (RT)-PCR. The
results showed that drought and salt stresses increased
the transcription level of OsBADH1 (Fig. 1A). Using
seedlings of rice japonica cv Nipponbare as an example,
we found that the expression of OsBADH1 was very
low under conditions without salt stress and detected
the increased transcripts when stresses were added.
However, a high ion concentration (0.5 M NaCl) inhibited
transcription of this gene. Similar results
were obtained when different rice varieties were used