At
the end of the experiment, 37 days after transplant, leaves
and
roots
were
sampled.
Both
for
leaves
and
root
an
aliquot
of
250
mg
of
shoot
or
root
tissue
was
ground
powder
with
liquid
nitrogen
with
a
mortar
and
pestle,
and
tissues,
into
put
tube.
2.5
mL
extraction
solvent
(2-propanol/H
O/HCl 37%; 2:1:0.002,
v/v/v)
was added to each tube. The tubes were shaken at a speed of
100
rpm
for
30
min
at
4
◦
2
C. 2.5 mL of dichloromethane was added
to
each tube, and then the samples were shaken for 30 min at 4