By previous investigations, the optimal condition was fixed. The
(L)-phenylalaninamide·HCl (6.0 mmol L−1) and CuSO4·5H2O (3.0
mmol L−1) dissolved in methanol/water (5/95, v/v) at pH=6.0 wasthe suitable mobile phase for chiral ligand exchange separation of
(D, L)-lactic acids (Fig. 10).