Development of aflatoxin-resistant cotton against Aspergillus flavus is handicapped by the lack of resistance
source in available germplasm. Genetic engineering warrants for identification of resistanceassociated
genes in cotton. As a first step toward this, we isolated 44 differentially expressed genes
(DEGs) in response to A. flavus infection, using annealing control primer system. Different functional
classification of the DEGs suggested a complex and multi-factorial plantefungus interaction. Eight DEGs,
including transcription factors, kinase, and downstream stress responsive genes, showed a tissue- and
time-dependent differences in their expression. The upregulated genes can be used as transgenes and/or
functional markers for breeding aflatoxin-resistant cottonseed.
Development of aflatoxin-resistant cotton against Aspergillus flavus is handicapped by the lack of resistancesource in available germplasm. Genetic engineering warrants for identification of resistanceassociatedgenes in cotton. As a first step toward this, we isolated 44 differentially expressed genes(DEGs) in response to A. flavus infection, using annealing control primer system. Different functionalclassification of the DEGs suggested a complex and multi-factorial plantefungus interaction. Eight DEGs,including transcription factors, kinase, and downstream stress responsive genes, showed a tissue- andtime-dependent differences in their expression. The upregulated genes can be used as transgenes and/orfunctional markers for breeding aflatoxin-resistant cottonseed.
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