Briefly, one ml of each
culture was harvested (5000 rpm, 5 min, 4 C), The sedimented
colonies were washed five times using phosphate buffered saline and suspended in 1.0 ml of sterilized water and the whole
genomic DNA was extracted by the CTAB method according
to the method explained by Sambrook and Fritscgh (1989).
The extracted DNA was suspended in DDW to be used for
PCR.