The ingredients used for each semi humid paste were ground in a laboratory mill (Thomas Willey Mod. 4, USA) to particles < 0.5 mm in diameter, and their moisture, total protein, total lipids, and ash contents were determined following methods established by A.O.A.C. (protocols 934.01, 976.05, 920.39 and 942.05, respectively). The diets were prepared by thoroughly mixing ingredients with purified water (200 ml to crab paste, squid paste, crab–squid paste and 125 ml to silage squid paste and silage–crab–squid paste) until a semi-moist diet resulted, with gelatin used as agglutinant (4.5%). Gelatine was first pre-gelatinised with cold water and after added to the dry ingredients during mixed with the rest of water.
The semi humid paste was prepared on a 2-week basis and stored at − 10 °C. Each diet's energy content was measured in a calorimetric pump (Parr@) calibrated with benzoic acid (Table 1). Octopuses were fed twice a day (9:00 and 14:00 h) with 30% living weight day− 1 for all diets (Quintana et al., 2011). Diets were separated into 200 mg portions and stored at − 10 °C until use. All the portions were placed over bivalve conchs that were offered to the octopus as a plate.
The ingredients used for each semi humid paste were ground in a laboratory mill (Thomas Willey Mod. 4, USA) to particles < 0.5 mm in diameter, and their moisture, total protein, total lipids, and ash contents were determined following methods established by A.O.A.C. (protocols 934.01, 976.05, 920.39 and 942.05, respectively). The diets were prepared by thoroughly mixing ingredients with purified water (200 ml to crab paste, squid paste, crab–squid paste and 125 ml to silage squid paste and silage–crab–squid paste) until a semi-moist diet resulted, with gelatin used as agglutinant (4.5%). Gelatine was first pre-gelatinised with cold water and after added to the dry ingredients during mixed with the rest of water.
The semi humid paste was prepared on a 2-week basis and stored at − 10 °C. Each diet's energy content was measured in a calorimetric pump (Parr@) calibrated with benzoic acid (Table 1). Octopuses were fed twice a day (9:00 and 14:00 h) with 30% living weight day− 1 for all diets (Quintana et al., 2011). Diets were separated into 200 mg portions and stored at − 10 °C until use. All the portions were placed over bivalve conchs that were offered to the octopus as a plate.
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