Optimization of steviol glycosides extraction from Stevia rebaudiana Bertoni leaf was carried out by investigating the effects of isopropyl alcohol concentration(60%,v/v), time (6–24 min), temperature (30 °C) and sonic power (300– 480 W) on extraction of rebaudioside A from Stevia rebaudiana leaves and decolorization of the extract by polymer (Separan AP30 and Resin ADS-7).