ฉันรักแปล•The LDH assay is a cytosolic enzyme present in many different
cell types
•Plasma membrane damage releases LDH into the cell culture media
•LDH catalyzes the conversion of lactate to pyruvate
•Diaphorase then uses NADH to reduce a tetrazolium salt (INT) to
a red formazan product that can be measured at 440nm
•Which is indicative of cytotoxicity