compared with reactor T5, there were two other absorption peaks of C C bending vibration for olefin at 698 cm−1 and 667 cm−1 for reactor T2, which maybe mainly due to C–C bond cleavage in carbohydrate; there was also one more absorption peak of C =C bending vibration at 667 cm−1 for reactor T4, compared with reactor T6; the results showed that sterilization could affect the biodegradation of carbohydrate only to a limited extent.