All active duty USAF members diagnosed with HIV infection have mandated visits at the San Antonio Military Medical Center (SAMMC) approximately every 6 months. Screening for HSV-1and -2 was performed at the first SAMMC visit after HIV diagnosisand periodically thereafter. All USAF members diagnosed with HIV between 1996 and 2012 with available HSV-1 and -2 serology datawere included. To evaluate the change in HSV seroprevalence overtime, patients were categorized into 2 groups by year of HIV diagnosis (1996–2004 and 2005–2012) similar to the recent NHANES study of HSV seroprevalence in the general US population [15].This retrospective study was approved by the SAMMC Institutional Review Board.