was separated from the solid residue (tomato seed) by centrifugation (Eppendorf 5804 R, Government Scientific Source, Reston, VA, USA) at 5000 rpm for 10 min at 4 C.
was separated from the solid residue (tomato seed) by centrifugation (Eppendorf 5804 R,Government Scientific Source, Reston, VA, USA) at 5000 rpm for 10 min at 4 C.