Development is the process by which a separation occurs in thin-layer chromatography by the differential migration of sample components in the direction of mobile phase flow. The movement of the mobile phase through the layer is driven by either capillary, pneumatic or electroosmotic forces. Most separations employ linear (edge-to-edge) with occasional use of radial (center-to-edgeor edge-to-center) development. Two-dimensional development is the result of two consecutive linear developments in orthogonal directions with removal of the mobile phase between developments, and uses standard linear developing chambers [16]. In any of these modes, continuous or multiple development can be used to improve the separation of mixtures with a narrow polarity range (continuous development) or wide polarity range(multiple development) [6,17–19]. Sandwich-type chambers for normal and continuous development were reviewed by Berezkin and Khrebtova [20] and Perry [21] has outlined the use of the short-bed continuous development chamber for optimized continuous development with the selection of variable development distances.