TLC
findings were in agreement with the data of qualitative
chemical tests. Ethyl acetate fraction (flavonoid
fraction) showed well marked 5 spots and acetone
extract (tannin fraction) showed well marked 6 spots,
shown in Fig. 1 and Table. 1. Topical formulations
(cream) have been developed containing flavonoid
(FF-I to III) and tannin fraction (TF-I to III) and the
developed formulation were examined for
antimicrobial activity against Propionibacterium acnes
and Staphylococcus epidermidis. MIC and MBC
values of flavonoid and tannin fraction against P.
acnes and S. epidermidis were determined by broth
dilution assay