Production assay
Strains ICFC 47/99, 748/12 and 751/12 were used in the production
assay.
2.5.1. Substrate preparation
Eucalyptus sp. sawdust (ES) and Populus sp. sawdust (PS) were
used as substrates, both pure with no supplementation. 25 × 45 cm
polypropylene bags were filled with 300 g of dry substrate per bag;
1% of CaCO3 powder was added. The moisture was adjusted to 70%
using tap water; bags finally reached 1000 g. They were autoclaved
for 2 h at 120 ◦C and 1.2 psi