Results
To evaluate whether the miRNAs extracted from the pelleted fraction
collected with EQ and UC methods are confined inside vesicles, experiments
with RNase A treatment were performed. All three miRNAs
(hsa-let-7a, hsa-miR-16 and hsa-miR-21) analysed with TaqMan® assays
were detected after the RNase A treatment of exosomal pellets isolated
with both methods, indicating that analysed miRNAs are confined
inside vesicles. miRNAs were not detected from samples that were
treated with RNase A after the RNA isolation from the pellets.