Metabolite analysis of Bacillus SE
Two antibacterial factors corresponding to autolysins and AHL-lactonases were screened. The percentage autolytic rate of the Bacillus SE strain was evaluated at pH 6 (31.35 ± 2.1), 6.5 (61.72 ± 3.4) and 8.5 (70.12 ± 2.7) in MES, phosphate, and Tris–HCl buffers respectively, using exponential phase cells grown in LB. The autolytic rates of peptidoglycan hydrolases of Bacillus SE strain were increased with increase in pH.
With regard to AHL-lactonases, the PCR product of corresponding aiiA gene was amplified in Bacillus SE strain as reported earlier (Lee et al. 2002) and it confirmed the presence of AHL-hydrolysing lactamases in Bacillus SE