A great advantage of the multiplex PCR method is that it enables
us to select the valuable genotypes in a single step, saving time,
effort, and resources. This method is ideal to quickly and efficiently
test the presence of resistance genes in the 899 individuals
of the VRH 3082-1-42 BC4 ×Kishmish vatkana BC5 cross, as well
as to study other hybrid families further on. It allows us to select
gene-pyramided genotypes and to perform routine progeny checks
through multiplex PCR for the joint presence of both the Run1 and
Ren1 genes.