The aim of this study was to efficiently utilize reed for both cellulase and biobutanol
production. The unprocessed cellulase blend produced under solid-state fermentation
using reed as the substrate showed a similar reducing sugar yield using Whatman filter
paper to the commercial enzyme blend (38.61%). Organosolv pretreatment method could
efficiently reduce hemicellulose (29.3%e14.6%) and lignin (17.2%e14.1%) content and increase
cellulose content (42.5%e62.3%) from reed. Enzymatic hydrolysis of organosolvpretreated
reed using the crude cellulase with enzyme loading of 25 FPU/g reed, 20%
solid content at 50 C and pH 5.5 resulted in a reed hydrolysate containing 40.01 g/L glucose
and 3.55 g/L xylose after 72 h. Fermentation of the hydrolysate medium by Clostridium
acetobutylicum produced 9.07 and 14.24 g/L of biobutanol and ABE with yield of 0.21 g/g and
0.33 g/g, respectively. This study proved that crude cellulase complex produced under solid
state fermentation and organsolv pretreatment can efficiently provide reed hydrolysate
that can be converted to biobutanol without any commercial cellulase usage