acclimationJuveniles of M. rosenbergii were obtained from the spawningof wild broodstock in a commercial hatchery located in Naihati,North 24 Parganas district of West Bengal, India and transportedin oxygenated polythene bag (pH 7.5, alkalinity 100 ppm as CaCO3,hardness 120 ppm as CaCO3) to the laboratory