Inhibition of the phytopathogens
The inhibition of the growth of test phytopathogens in agar well diffusion technique was expressed in terms of percentage inhibition. The crude culture filtrate showed the maximum inhibition after 96 hours of fermentation at 37oC with the maximum inhibition of Fusarium oxysporum upto 53.29% and no inhibition of the Phytophthora infestans.
The percentage inhibition is calculated as (R−r)/R × 100, where R is mycelial growth away from well filled with culture filtrate (the maximum growth of the fungal mycelia), r is mycelial growth towards the well filled with the culture filtrate. Different inhibition pattern of the culture filtrate is given in the table 2 below.
The crude culture filtrate showed the highest percentage inhibition to Fusarium oxysporum (53.29%) at 96 hours of incubation, lowest to Exserohilum turcicum and no inhibition to Phytophthora infestans.