They were delivered to the laboratory and individuals were opened within the first 4 h post-mortem headed and gutted and then, the fish was filleted, cut into pieces (dimensions:10 × 5 × 1,5; weight range: 50–55 g) which were extracted from the dorsal part of the fish, and placed in flexible high-density polyethylene bags and vacuum-sealed, and maintained under chilling conditions (~30– 60 min) in a refrigerated room (4 °C) until further HHP processing