3.Results
3.1 Effect of intraduodenal administration of LEM on Splenic-SNA in urethane-anesthetized rats
Typical reordings of Splenic-SNA in urethane-anesthetized rats
following intraduodenal injection of 1 ml of water and 1 ml of the LEM solution are shown in Fig. 1a Means+SEMs of the percentage change from the 0-min values of the Splenic-SNA of 3 rats are shown in Fig. 1b. A Slight increase was observed from 25 min after the intraduodenal injection of water (Fig. 1b). In contrast, an intraduodenal injection of 1 ml of the LEM solution gradually and markedly lowered Splenic-SNA, with the lowest level being 60.2+14.3% at 60 min after the injection (Fig. 1b). The statistical significance of the difference between the groups with regard to the values of Splenic-SNA 5-60 min after the administration of the LEM solution and water was analyzed by ANOVA, and the difference was found to be statistically significant (P