tPomegranate fruit (Punica granatum L.) production and consumption has increased recently due toincreasing scientific evidence on its high content of health beneficial compounds. This study wasconducted to investigate the phytochemical contents and antioxidant activity of pomegranates (cv. Won-derful) as affected by fruit maturation and growing location. High performance liquid chromatography(HPLC) coupled with liquid chromatography mass-spectrometry (LC–MS) and liquid chromatographymass-spectrometry electroscopy (LC–MSE) were used to analyse phenolic composition at differentmaturity stages. Catechin, epicatechin and naringin were the most dominant flavonoids irrespectiveof maturity and altitude, while gallic acid was the dominant phenolic acid. The concentrations of totalphenolics and total tannins as well as radical scavenging activity (RSA) in DPPH assay declined as maturityadvanced while ferric reducing antioxidant power (FRAP), total anthocyanin, total flavonoid and vitaminC increased significantly (P < 0.01). There was a significant and negative correlation (r = −0.64) betweentotal phenolic content and antioxidant activity in the FRAP assay. Principal component analysis (PCA)showed that fruit grown in area with lower altitude were associated with higher bioactive compoundsat full ripe stage. Furthermore, PCA plot also revealed that fruit growing location had a significant andprominent impact on the bioactive compounds than maturity status.