True protein methods
1.Colorimetric/ dry binding technique
-Lowry method, Bio-rad, Bradford's assay etc.
-Involve reactions between peptide N, or acidic or basidic aas with a dye
-Measure soluble/available N
-Appropriateness for forage with fiber/tannin bound N
-Result may depend on particle size and peptide size, presence of reagents that interfere with the reaction