(A) Cells isolated from brains were stained for CD45, CD11b, Ly6G, and Ly6C for flow cytometric analysis. Representative pseudocolor plots demonstrate the gating strategy used to identify resident CD11bposCD45lo microglia (lower right quadrant) and recruited CD11bposCD45hi myeloid cells in the upper right quadrant, from which the Ly6Chi monocytes were further separated (right plot) from Ly6Gpos neutrophil granulocytes.