The yeast and mold strains to be tested were respectively grown on PDA for 48–72 h and for 7–12 days at 25 °C. To evaluate the antagonistic activity against B. cinerea, a loopful of yeasts strains was streaked orthogonally from the center of Petri dish, containing PDA pH 6.0 and pH 4.5 media. Three plates for each yeast strain and pH were used. After incubation at 25 °C for 48 h, mycelial discs (5-mm square plug) of B. cinerea were placed on agar plates 3 cm away from yeast inoculum. A control dish only inoculated with gray mold was also prepared. At the end of the incubation period (12 days at 25 °C) radial growth reduction was calculated in relation to growth of the control as follows: %I = (C − T/C) × 100, where %I represented the inhibition of radial mycelial growth, C was radial growth measurement in control and T was the radial growth of the pathogen in the presence of yeast strains. The experiments were repeated three times.