2. Material and methods
2.1. Materials
All solvents were obtained commercially and appropriately
purified, if necessary. Carbon dioxide (99.98 %) was from Air Liquide.
Squalene (98% by weight) and oleic acid (technical grade,
79.9% by mass; other major fatty acids present included myristic
acid (C14:0) at 2.0%, palmitoleic acid (C16:1) at 5.2%, palmitic acid
(C16:0) at 5.6%, stearic acid (C18:0) at 1.0%, and linoleic acid
(C18:2) at 6.4%) were supplied by Sigma Aldrich. Choline hydrogen
carbonate was synthesized following the procedure described in
the literature [11]. The olive oil deodorizer distillate used in the
experiments was gently provided by Sovena S.A. (Lisbon, Portugal).