Chromatography analysis for the quantification of the chemical
marker gallic acid in S. brasiliensis was conducted on a Shimadzu®
HPLC model LC-10 controlled by Class VP Software and with quaternary
pumps (LC-10AD), an on-line degasser (DGU-14A), and an
injection valve (Rheodyne® LLC) with a 20 l loop. The equipment
included a UV–Vis detector (SPD-10A). The column used was a
Phenomenex RP-Gemini NX C18 (250 mm
×
4.6 mm, 5 m) and it
was protected by a pre-column Gemini NX C18 (10 mm
×
4 mm).