Another possible reason
could be nutrient rich diet being fed to females
during the pregnancy period and the same (nutrient
rich) food is being utilized by the parasites thereby
resulting in more growth. It was also observed that
fixation had a direct effect on the size of the body
and other body organs as the parasites fixed in too
hot or too cold fixative shrink to different lengths as
compared to those fixed in alcohol with optimum
temperature.