Polyaniline (PANI) with/without polystyrene (PS), was successfully manufactured in the occurrence ofdispersed pulp fibers via the oxidative polymerization reaction of aniline monomer to produce conduc-tive paper sheets containing PANI, PANI/PS composites. Additionally, sliver nitrate (Ag-NO3) was addedby varied loadings to the oxidative polymerization of aniline monomer to provide sliver nanoparticles(Ag-NPs) emptied into the prepared paper sheets. The prepared paper sheets were examined using scan-ning electron microscopy (SEM), X-ray diffraction (XRD) and infrared spectroscopy (IR), the mechanicalproperties of the prepared paper sheets were evaluated. Moreover, the electrical conductivity and biolog-ical studies such as cellulases assay, Microorganism & culture condition and detection of the released ofAg-NPs were evaluated. Furthermore, the prepared paper sheets were displayed good antibacterial prop-erties contrary to gram positive and gram negative bacteria. Consequently, the prepared paper sheet maybe used as novel materials for packaging applications.