Histograms showing the relative expression profiles of PmVtg1 mRNA (50 ng cDNA
tempate) during ovarian development of intact and unilateral eyestalk-ablated P. monodon
broodstock. The expression level was measured as an absolute copy number of PmVtg1 and
normalized by that of EF-1α from the same shrimp. Each histogram corresponds to a
particular ovarian stage. The same letters above bars indicate that the expression levels
were not significantly different (P N 0.05). JN = cultured juveniles; I–IV = stages I–IV
ovaries of intact or eyestalk-ablated broodstock; PS = post-spawning intact broodstock