According Kilikian and Pessoa [4], the addition of ethanol in a single application can lead to denaturation of enzymes by forming ‘‘pockets’’ of ethanol in the solution, even if the temperature is below the critical temperature (>10.0 C).
According Kilikian and Pessoa [4], the addition of ethanol in a singleapplication can lead to denaturation of enzymes by forming ‘‘pockets’’of ethanol in the solution, even if the temperature is below thecritical temperature (>10.0 C).