mutant lines had a reduced transcript level for BnFAD3 which catalyzes
the conversion of C18:2 into C18:3. The expression of BnFAE1,
involved in erucic acid biosynthesis, was only suppressed in the M1
and M2 lines (Fig. 5).
Expression studies of five representative Brassica OLEOSIN genes
(Jolivet et al., 2009) revealed suppression of OLEOSIN S-5 in all
BnFUS3 mutants. The expression level of OLEOSIN S-2 was reduced
in M1 and M2 and S-3 expression was reduced only in M1
(Supplemental Fig. 8).