Fig. 3. Agarose gel electrophoresis of PCR amplification products demonstrating the sensitivity of the 261F and 261R primer set. The detection
limit of primer 261F/R is 100 copies of plasmid clone WS-1 containing the 261 bp fragment. Lane M: 1 kb molecular weight ladder. Lanes 2–
13: 10-fold serial dilutions of WS-1 plasmid DNA (1010 to 1 copies, respectively). Lane 13: no-template (NT) control. The data is representative of two independent experiments