In the first phase, a non-renewal static toxicity bioassay was done for salinity range finding as described by Peltier and Weber(1985).
Juveniles of M. rosenbergii (length: 6.98 ± 0.67 cm; weight:4.05 ± 0.84 g) were directly transferred to 0, 5, 10, 15, 20, 25 and 30 ppt saline water.
Desired salinities were achieved by mixing freshwater with brine water collected from salt pan (>100 ppt salinity).
The experimental system consisted of 10 L glass aquarium stocked with ten juveniles/aquarium for 96 h with three replicates.