Five types of low-value fish protein hydrolysates (FPH) and ferrous-chelating peptides
[Fe(II)–FPH] were prepared from Pacific mackerel, Spanish mackerel,
hairtail, Japanese anchovy and horse mackerel. The hydrolysis degrees of FPH and
ferrous chelation percentages of all types Fe(II)–FPH were calculated. The antioxidant
activities were measured using 2,2-diphenyl-1-picryl-hydrazyl (DPPH)-
scavenging activity and hydroxyl radical-scavenging activity. All FPH samples
demonstrated low antioxidant activity. After ferrous chelation, the DPPHscavenging
activities of Spanish mackerel-Fe(II)–FPH and hairtail-Fe(II)–FPH
were significantly higher (P < 0.05 and P < 0.01, respectively) than other FPH, and
the hydroxyl radical-scavenging activity of hairtail-Fe(II)–FPH was significantly
higher (P < 0.05) than that of hairtail-FPH. Among all the tested, hairtail-Fe(II)–
FPH showed the highest ferrous chelation percentage and antioxidant activity. The
ferrous-chelating peptide fractions recovered from low-value fish, especially small,
low-value hairtail, can be used as a source of natural antioxidants for food
products.
Five types of low-value fish protein hydrolysates (FPH) and ferrous-chelating peptides[Fe(II)–FPH] were prepared from Pacific mackerel, Spanish mackerel,hairtail, Japanese anchovy and horse mackerel. The hydrolysis degrees of FPH andferrous chelation percentages of all types Fe(II)–FPH were calculated. The antioxidantactivities were measured using 2,2-diphenyl-1-picryl-hydrazyl (DPPH)-scavenging activity and hydroxyl radical-scavenging activity. All FPH samplesdemonstrated low antioxidant activity. After ferrous chelation, the DPPHscavengingactivities of Spanish mackerel-Fe(II)–FPH and hairtail-Fe(II)–FPHwere significantly higher (P < 0.05 and P < 0.01, respectively) than other FPH, andthe hydroxyl radical-scavenging activity of hairtail-Fe(II)–FPH was significantlyhigher (P < 0.05) than that of hairtail-FPH. Among all the tested, hairtail-Fe(II)–FPH showed the highest ferrous chelation percentage and antioxidant activity. Theferrous-chelating peptide fractions recovered from low-value fish, especially small,low-value hairtail, can be used as a source of natural antioxidants for foodproducts.
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