Amylose content of the samples was determined by colorimetric measurement of the blue amyloseeiodine complex
In summary, 100 mg of sample were weighed into a 100 mL volu-metricflask and mixed with 1 mL ethanol
and 9 mL of 2 mol/L NaOH. The samples were diluted and the iodine solution was added.
After 10 min incubation at room temperature, the absorbance at 620 nm was analysed with a spectrophotometer and the amylose content was calculated based on the standard curve.