Laser diffraction was used to determine both the oil droplet size of emulsions after the first emulsification step (single O/W emulsions) and water droplet size of emulsions after the second emulsification step (double O/W/O emulsions). For the characterization of double emulsions, it was assumed that the possible presence of inner oil droplets does not disturb the measurement signal received from outer water droplets. Fig. 1 shows the DSD of O/W/O emulsions as well as the DSD of single O/W emulsions that were used to prepare the multiple emulsions. For O/W emulsions, it can be seen that higher emulsifier content leads to the stabilization of smaller oil droplets when the emulsification process is kept constant. The DSD of water droplets in O/W/O after stirring differs significantly. The double emulsions prepared with lower hydrophilic emulsifier content display three times larger water droplet sizes. Harsh processing of double emulsions leads to a size reduction of aqueous droplets that is more pronounced in emulsions with low hydrophilic emulsifier content. After 3 min of processing in the colloid mill, the DSD of water droplets is almost equal for both types of prepared multiple emulsions.