3.9. Measurements of galactinol and raffinose contents
Plants of transgenic lines (R3 and R5) and the non-transformed control plants were treated at 4 °C for 24 h. Then set at normal conditions for 3 h for recovery. Leaves were collected and used as experimental materials for galactinol and raffinose content measurements. Results shown in Fig. 10 indicated that in normal conditions neither galactinol nor raffinose was detectable, but they were detected obviously in transgenic plants. That is resulted from the expression of the transformed AmGS gene; and that in cold-stress, there were low contents of endogenous galactinol and raffinose were found in untransformed control plants, but the levels were much lower than that of the transgenic plants.