2.2. Microalgae cultivation
2.2.1. Shake flasks
Three different microalgae species were cultured. The freshwater
microalgae species Chlorella kessleri (strain UTEX2229) and Chlorella
vulgaris (strain CCAP211/19) were obtained from the collection of
algae at the University of Nantes; and the marine microalgae species
N. oculata, from the Alphabiotech collection (Asserac, France).
Themicroalgae were first inoculated in shake flasks. The freshwater
microalgae species in 250 mL Erlenmeyer flask containing a modified
Bold BasalMedium(BBM)and themarine microalgae species, in filtered
and sterilised seawaterwith salinity adjusted at 25‰and enrichedwith
Conway medium (3 mL/L of seawater). The detailed composition of the
modified BBM and Conway medium is given in Pruvost et al. [16,17