Using the stems and leaves of banana as raw materials to make methane by anaerobic fermentation. Before the anaerobic process the saccharifying enzyme hydrolysis of raw materials is the linchpin. Enzyme hydrolysis is a new technology, which mainly use cellulase to hydrolyze and ferment cellulose to generate methane [1] [2]. Enzyme hydrolysis method belongs to biological method and it has the characteristics of reacting at room temperature, low power consumption, nice selectivity of enzyme, high sugar yield, simple process and no pollution [3] [4]. Select cellulose to hydrolyze the material and confirm and optimize the conditions of enzyme hydrolysis saccharification process. The enzyme hydrolysis saccharification process are affected by many