Initiation: For callus initiation, a stem explant of
bilimbi or aromatic chilli was placed horizontally
for 3 weeks on the surface of: (a) a PLA and
(b) a PVDF nanofibre disc, each of 2.54 cm cut
out from a nanofibre mat using a cork borer. The
medium used in both treatments for bilimbi or aromatic
chilli was liquid basal MS medium (3 ml)
containing 2 mg/l -naphthalene acetic acid (NAA) or
2,4-dichlorophenoxy acetic acid (2,4-D), respectively.
Subculture: For the callus subculture experiment,
stem explants of bilimbi or aromatic chilli were first
cultured for 3 weeks on basal MS medium supplemented
with 2 mg/l NAA or 2,4-D, respectively,
gelled with 0.8% (w/v) agar (Fig. 2). The callus
thus initiated was used for subculture in the same 2
aforementioned treatments (a) and (b) for 3 weeks.
A piece of subcultured callus (0.8–1 cm width0.8–
1 cm length) was placed on an appropriate nanofibre
mat.