An in vitro shoot culture method has been developed for propagating C. wilsoniana, an important biofuel plant.
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Explant browning can be suppressed by culturing on DKW medium supplemented with PVP.
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More than 4 axillary shoots are induced per explant cultured on DKW medium containing 300 mg L−1 PVP, 0.5 mg L−1 zeatin and 0.1 mg L−1 IBA.
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This method can lead to rapid commercial propagation of high yield cultivars of C. wilsoniana.