The percentages of mass retention of total isoflavones by the soaked soybean (RISS, Eq. (2)), the mass loss of total isoflavones into the soaking medium (LIRS, Eq. (3)), and the mass degradation of total isoflavones in the systems (DISY, Eq. (4)) were expressed (Table 1) on the basis of the mass balance of total isoflavones (Eq. (5)) of the systems in relation to the mass of isoflavones from the whole soybeans before soaking. It is noteworthy that DISY was considered only when there was a significant difference (p < 0.05) between the contents of total isoflavones in the system compared to that in the whole soybeans. Likewise, RISS was considered as 100% when the content of total isoflavones in the soaked grains was not significantly different (p > 0.05) in relation to the raw soybean. In addition, the masses of the soaked soybeans throughout the soaking were also reported