a b s t r a c t
The antiglycoxidative properties of theanine (TEF) and polyphenols enriched fractions (PEF) prepared
from tea dust were tested in a model system composed of bovine serum albumin (BSA) and methylglyoxal
(MGO). PEF caused a decrease in available free amino groups of BSA in presence and absence of MGO,
suggesting the simultaneous occurrence of glycoxidation reaction and phenols-protein interaction. The
presence of PEF and TEF inhibited formation of fluorescent advanced glycation end-products (AGEs).
Moreover, theanine (TB) and polyphenol-enriched bread (PB) were formulated. A significant increase in
free amino groups was observed in TBs with a dose–response effect, while addition of PEF in bread produced
a significant decrease (p < 0.05). PEF efficiently reduced fluorescent AGE formation in breads compared
with TEF. The results are in line with the simplified model systems. PEF used as food ingredient
allows obtaining a tasty food possessing health promoting properties and lower content of potential
harmful compounds (AGEs).